Chapter 27 Assembly functional annotation

In this section we will carry out carry out functional annotation of our MAGs/bin/bins. There are many different ways to carry this. In this tutorial we will use 2 tools:

• Bakta: Annotation of Bacterial genomes and plasmids.
• MinPath: Prediction of metabolic pathways.
  • Include MetaCyc and KEGG pathways.

Conda Environment: Go back to your shotgun_meta terminal (or create a new one and use . useshotgun).

Taxonomic annotation

Taxonomic annotation of bins can be carried out with Kraken2. As we have already done this for the reads and taxonomic results from read and assembly approaches have similar performances we will not cover it here.