Metabarcoding for diet analysis and environmental DNA - supplemental material

Katy Maher, Helen Hipperson and Ewan Harney

2025-05-19

Chapter 1 Introduction

This bookdown is supplemental to the main one. It contains some additional code and examples of tools that may prove useful in addition to the core DADA2 pipeline.

Ensure you have the metabarcoding environment activated for using all of these materials. As a reminder, the command to do this (if you are not still in the R session) is:

. usemetabarcoding

Table of contents

Normalising sequence data	Exporting data
Classifying ASVs with GenBank and BLAST	Assigning taxa with MEGAN
Further blast and megan approaches	Summary

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